-acetyl derivative. This result's in step with the formation or collapse from the tetrahedral intermediate as the rate restricting phase during the catalytic mechanism of NagA.
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m is not really restricted by the Affiliation price continuous for that development from the NagA-substrate elaborate. These benefits (together with the improved amount of response of compound 6 relative to compound one) are only consistent with the summary that NagA is limited by either the formation or breakdown of the tetrahedral intermediate.
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The second ionization noticed during the log kcat/Km profile could originate from other residues within the Lively web-site of NagA but it could also arise from the protonation of your substrate alone Considering that the phosphate moiety incorporates a p
a could be the acid dissociation consistent. pH-price profiles that showed the lack of catalytic exercise at substantial and small values of pH were being healthy to equation 4, the place K
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